Perform differential analysis between subgroups at each partition
Source:R/runDGEmods.R
runDGEmods.Rd
Adds differential analysis results to the output of K2tax().
Usage
runDGEmods(
K2res,
DGEmethod = NULL,
DGEexpThreshold = NULL,
vehicle = NULL,
variables = NULL,
block = NULL,
logCounts = NULL
)
Arguments
- DGEmethod
Character. Method for running differential gene expression analyses. Use one of either 'limma' (default) or 'mast'.
- DGEexpThreshold
Numeric. A value between 0 and 1 indicating for filtering lowly expressed genes for partition-specific differential gene expression. Proportion of observations with counts > 0 in at least one subgroup at a specific partition.
- vehicle
The value in the cohort variable that contains the ID of observation to use as control. Default NULL if no vehicle to be used.
- variables
Character. Columns in meta data of 'object' to control for in differential analyses.
- block
Character. Block parameter in limma for modeling higherarchical data structure, such as multiple observations per individual.
- logCounts
Logical. Whether or not expression values are log-scale counts or log normalized counts from RNA-seq. Default is TRUE.
References
Reed ER, Monti S (2021). “Multi-resolution characterization of molecular taxonomies in bulk and single-cell transcriptomics data.” Nucleic Acids Research. doi:10.1093/nar/gkab552 , https://pubmed.ncbi.nlm.nih.gov/34226941/. Ritchie ME, Phipson B, Wu D, Hu Y, Law CW, Shi W, Smyth GK (2015). “limma powers differential expression analyses for RNA-sequencing and microarray studies.” Nucleic Acids Research, 43(7), e47--e47. ISSN 1362-4962, 0305-1048, doi:10.1093/nar/gkv007 , http://academic.oup.com/nar/article/43/7/e47/2414268/limma-powers-differential-expression-analyses-for. Benjamini Y, Hochberg Y (1995). “Controlling the False Discovery Rate: A Practical and Powerful Approach to Multiple Testing.” Journal of the Royal Statistical Society: Series B (Methodological), 57(1), 289--300. ISSN 00359246, doi:10.1111/j.2517-6161.1995.tb02031.x , http://doi.wiley.com/10.1111/j.2517-6161.1995.tb02031.x.